Microscopes
- Zeiss AxioImager Microscope:
- Upright microscope for wide field differential interference contrast (DIC), polarized light and fluorescence microscopy
- Color camera for immunohistochemical (IHC) imaging and monochrome camera for sensitive fluorescence imaging
- Controlled by Zeiss AxioVision software
- Objectives: 5x, 10x, 20x, 40x oil, 63x oil, 100x oil
- Fluorescent filters: DIC, DAPI, CFP, GFP, YFP, DsRed, TexRed, Cy5
- Zeiss AxioObserver Z1 Microscope:
- Inverted microscope for DIC and fluorescence microscopy of cell cultures in small dishes and multi-well plates
- Heated stage insert for live cell time-lapse imaging
- Controlled by Zeiss AxioVision software
- Objectives: 2.5x, 10x, 20x, 40x oil, 63x oil
- Fluorescent filters: DIC, DAPI, GFP, DsRed, PI, Cy5
- Zeiss LSM 880 Confocal Microscope with Airyscan:
- Laser scanning confocal light microscope for fluorescence and differential interference contrast microscopy
- Heated stage insert for live cell time-lapse imaging
- Controlled by Zeiss ZEN 2 software
- Objectives: 10x, 20x, 40x oil, 63x water, 63x oil, 100x oil
- Excitation lasers (nm): 405, 458, 488, 514, 561, 594, 633
- Zeiss ELYRA S1 Super-Resolution Microscope:
- Structured illumination microscopy (SIM) and localization microscopy (PALM/STORM)
- Heated stage insert for live cell imaging
- Controlled by Zeiss ZEN 2 software
- Objectives: 10x, 20x, 64x oil, 100x oil
- Excitation lasers (nm): 405, 488, 561, 642
- FEI Tecnai F20 200 keV Transmission Electron Microscope (TEM):
- Electron microscope with field emission gun and 4 K FEI Eagle camera
- Controlled by FEI software with SerialEM software for tomography
- Workstation PC for image processing
- Full Zeiss ZEN software for confocal and super-resolution image processing
- Zeiss AxioVision
- FIJI
- eTomo and 3dmod for TEM tomography processing
TEM Sample Preparation Equipment
- Leica EMPACT2/AFS2:
- Automated high pressure freezing and freeze substitution for electron microscope sample preparation
- Leica Ultracut 7 Microtome with Cryo-attachment:
- Electronic controlled microtome for sectioning plastic embedded and cryofrozen samples for electron microscopy
- Diatome diamond knives for ultra thin plastic and cryo sections
- Glass knife for cutting tomography sections
- FEI Vitrobot:
- Electronic controlled plunge freezing of samples for electron microscopy
Fees and Terms of Service
- Availability:
- The Digital Microscopy Core is a shared research facility available to all investigators in the state of Arkansas.
- The facility is accessible to trained users 24 hours a day, 7 days a week.
- Training and technical assistance is available 9:00 a.m. – 5:00 p.m. Monday through Friday with prior scheduling.
- Access to scheduling and sample submission through iLab core management
- Scheduling:
- Trained users may reserve time using the online scheduling.
- Training and technical assistance scheduled with technician approval.
- Priority for reserving the microscopes is given to BRIN/INBRE students and the six laboratories known as “SIG” users.
- Other users may reserve instruments on a first come, first serve basis when priority users are not scheduled.
- Failing to arrive or call within 20 minutes of their scheduled reservation, the reserved time will be forfeited
- TEM Sample processing:
- EM sample submission done through iLab request for services.
- Limited to 48 hour prior notice with arranged exceptions
- Fees: Current fees can be viewed here.
- Acknowledgement: All users agree to acknowledge use of the Digital Microscopy Core in scholarly work and to provide a copy of published work.
Contact Information and Request for Services
Brian Storrie, Ph.D. – Director
Department of Physiology & Biophysics, College of Medicine
Jeff Kamykowski – Lab Contact
(501) 526-7290
Biomedical Research Building II, Room 136-2
4301 West Markham Street, #505
Little Rock, AR 72205