Microscopes

• Zeiss AxioImager Microscope:
  • Upright microscope for wide field differential interference contrast (DIC), polarized light and fluorescence microscopy
  • Color camera for immunohistochemical (IHC) imaging and monochrome camera for sensitive fluorescence imaging
  • Controlled by Zeiss AxioVision software
  • Objectives: 5x, 10x, 20x, 40x oil, 63x oil, 100x oil
  • Fluorescent filters: DIC, DAPI, CFP, GFP, YFP, DsRed, TexRed, Cy5
• Zeiss AxioObserver Z1 Microscope:
  • Inverted microscope for DIC and fluorescence microscopy of cell cultures in small dishes and multi-well plates
  • Heated stage insert for live cell time-lapse imaging
  • Controlled by Zeiss AxioVision software
  • Objectives: 2.5x, 10x, 20x, 40x oil, 63x oil
  • Fluorescent filters: DIC, DAPI, GFP, DsRed, PI, Cy5
• Zeiss LSM 880 Confocal Microscope with Airyscan:
  • Laser scanning confocal light microscope for fluorescence and differential interference contrast microscopy
  • Heated stage insert for live cell time-lapse imaging
  • Controlled by Zeiss ZEN 2 software
  • Objectives: 10x, 20x, 40x oil, 63x water, 63x oil, 100x oil
  • Excitation lasers (nm): 405, 458, 488, 514, 561, 594, 633
• Zeiss ELYRA S1 Super-Resolution Microscope:
  • Structured illumination microscopy (SIM) and localization microscopy (PALM/STORM)
  • Heated stage insert for live cell imaging
  • Controlled by Zeiss ZEN 2 software
  • Objectives: 10x, 20x, 64x oil, 100x oil
  • Excitation lasers (nm): 405, 488, 561, 642
• FEI Tecnai F20 200 keV Transmission Electron Microscope (TEM):
  • Electron microscope with field emission gun and 4 K FEI Eagle camera
  • Controlled by FEI software with SerialEM software for tomography
  • Workstation PC for image processing
  • Full Zeiss ZEN software for confocal and super-resolution image processing
  • Zeiss AxioVision
  • FIJI
  • eTomo and 3dmod for TEM tomography processing
    TEM Sample Preparation Equipment
• Leica EMPACT2/AFS2:
  • Automated high pressure freezing and freeze substitution for electron microscope sample preparation
• Leica Ultracut 7 Microtome with Cryo-attachment:
  • Electronic controlled microtome for sectioning plastic embedded and cryofrozen samples for electron microscopy
  • Diatome diamond knives for ultra thin plastic and cryo sections
  • Glass knife for cutting tomography sections
• FEI Vitrobot:
  • Electronic controlled plunge freezing of samples for electron microscopy

Fees and Terms of Service

• Availability:
  • The Digital Microscopy Core is a shared research facility available to all investigators in the state of Arkansas.
  • The facility is accessible to trained users 24 hours a day, 7 days a week.
  • Training and technical assistance is available 9:00 a.m. – 5:00 p.m. Monday through Friday with prior scheduling.
  • Access to scheduling and sample submission through iLab core management
• Scheduling:
  • Trained users may reserve time using the online scheduling.
  • Training and technical assistance scheduled with technician approval.
  • Priority for reserving the microscopes is given to BRIN/INBRE students and the six laboratories known as “SIG” users.
  • Other users may reserve instruments on a first come, first serve basis when priority users are not scheduled.
  • Failing to arrive or call within 20 minutes of their scheduled reservation, the reserved time will be forfeited
• TEM Sample processing:
  • EM sample submission done through iLab request for services.
  • Limited to 48 hour prior notice with arranged exceptions
• Fees: Current fees can be viewed here
• Acknowledgement: All users agree to acknowledge use of the Digital Microscopy Core in scholarly work and to provide a copy of published work.

Contact Information and Request for Services

Brian Storrie, Ph.D. – Director
Department of Physiology & Biophysics, College of Medicine

Jeff Kamykowski – Lab Contact
(501) 526-7290

Biomedical Research Building II, Room 136-2
4301 West Markham Street, #505
Little Rock, AR 72205